Optimal proportioning design of culture medium for corynebacterium glutamicum q 谷氨酸生產菌培養(yǎng)基最優(yōu)配比設計
Construction of corynebacterium glutamicum e . coli shuttle promoter - probe vector 大腸桿菌穿梭型啟動子探測載體構建
A combined metabolic mutated strain ( ys - c ) was get by traditional filter method from corynebacterium glutamicum ( ys ) . this mutant is threonine auxotrop , aec and ahv resistant , and it can grow in the medium which contants sca as the only source of carbon , and the mutant can endure hight leveal of leu . the mutanted strain can concentrate lysine . the concentration of lysine leveal was 1 . 20g / l 運用傳統(tǒng)育種手段篩選到谷氨酸棒桿ys的多重代謝突變組合菌株ys - c 。該菌株為蘇氨酸營養(yǎng)缺陷型,具有對賴氨酸的結構類似物aec和ahv的抗性,并且能以琥珀酸為唯一碳源生長,能夠耐受高濃度亮氨酸。